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Image Search Results
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Protein-tyrosine kinase Syk expressed in human nasal fibroblasts and its effect on RANTES production.
doi: 10.4049/jimmunol.166.1.538
Figure Lengend Snippet: FIGURE 5. LPS induced phosphorylation of Syk and JNK1. A, The fibroblasts (case 9) were cultured without LPS (lane 1), or with LPS for 2 min (lane 2), for 10 min (lane 3), and for 30 min (lane 4). The sample was immunoprecipitated with anti-phosphotyrosine Ab and blotted with anti- Syk Ab. The same amount of lysate (30 mg) before immunoprecipitation was applied to each lane and blotted with anti-Syk Ab. The positions of phosphorylated Syk (P-Syk) and total amount of Syk were indicated to the right with the arrows. B, The same amount of lysate from the cells after LPS stimulation was applied to each lane, as shown above, and blotted with anti-phosphorylated JNK Ab (top), and with anti-JNK1 Ab (bottom). The po- sitions of phosphorylated JNK1 (P-JNK1) and total amount of JNK1 were indicated to the right with the arrows. The lanes are the same as above.
Article Snippet: Anti-Syk mAbs (4D10, C20, N19), anti-Lyn Ab, anti-c-Jun N-terminal kinase 1 (JNK1)3 Ab,
Techniques: Phospho-proteomics, Cell Culture, Immunoprecipitation
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Protein-tyrosine kinase Syk expressed in human nasal fibroblasts and its effect on RANTES production.
doi: 10.4049/jimmunol.166.1.538
Figure Lengend Snippet: FIGURE 6. Decreased Syk expression attenuated JNK1 activation. Na- sal fibroblasts (case 9) were transfected with 10 mM Syk antisense (lane 3) or 10 mM Syk sense (lane 2) or no oligodeoxynucleotide (lane 1) for 6 h, and then stimulated with 5 mg/ml LPS for 10 min. The same amount of lysate was applied to each lane and blotted with anti-phosphorylated JNK Ab, anti-JNK1 Ab, and anti-Syk Ab. The position of phosphorylated JNK1 (P-JNK1) and the expression of JNK1 or Syk were indicated to the right with the arrows.
Article Snippet: Anti-Syk mAbs (4D10, C20, N19), anti-Lyn Ab, anti-c-Jun N-terminal kinase 1 (JNK1)3 Ab,
Techniques: Expressing, Activation Assay, Transfection